Abundant human anti-Galα3Gal antibodies display broad pathogen reactivity.
|Title||Abundant human anti-Galα3Gal antibodies display broad pathogen reactivity.|
|Publication Type||Journal Article|
|Year of Publication||2020|
|Authors||Jensen, JMagnus Ber, Petersen, MSteen, Ellerman-Eriksen, S, Møller, BKuno, Jensenius, JChristian, Sørensen, UBSkov, Thiel, S|
|Date Published||2020 03 12|
|Keywords||Antibodies, Disaccharides, Enzyme-Linked Immunosorbent Assay, Escherichia coli, Escherichia coli Infections, Host-Pathogen Interactions, Humans, Immunoglobulin G, Sepsis|
Antibodies of the IgG class to terminal Galα3Gal (IgG anti-αGal) is abundant in human plasma and are reported to bind most sepsis-causing Gram-negative bacteria. However, these seminal findings, made more than two decades ago, have not been reexamined. Our aim was to assess IgG anti-αGal´s pathogen reactivity. We affinity purified IgG anti-αGal from a therapeutic grade normal human IgG pool applying two rounds of positive selection with Galα3Gal-coupled beads and included removal of column matrix reactive antibodies. The purified antibodies were rigorously characterized in terms of specificity and purity in various solid-phase immunoassays. We used flow cytometry to study reactivity against 100 consecutive clinical isolates diagnosed as cause of sepsis in humans. We found that the purified IgG anti-αGal displays high specificity for Galα3Gal. Also, IgG anti-αGal at 5 mg/L bound 56 out of 100 pathogens with predilection for Gram-positive bacteria binding 39 out of 52 strains. We confirm that although IgG anti-αGal comprise a small fraction of the human antibody pool (~0.1%), these antibodies targets an impressively large part of pathogens causing invasive disease.
|Alternate Journal||Sci Rep|
|PubMed Central ID||PMC7067764|