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Capsular gene typing of Streptococcus agalactiae compared to serotyping by latex agglutination.

TitleCapsular gene typing of Streptococcus agalactiae compared to serotyping by latex agglutination.
Publication TypeJournal Article
Year of Publication2013
AuthorsYao, K, Poulsen, K, Maione, D, C Rinaudo, D, Baldassarri, L, Telford, JL, Sørensen, UBSkov, Kilian, M
Corporate AuthorsMembers of the DEVANI Study Group
JournalJ Clin Microbiol
Date Published2013 Feb
KeywordsAnimals, Bacterial Capsules, Cattle, Female, Flow Cytometry, Genetic Loci, Humans, Latex Fixation Tests, Molecular Typing, Pregnancy, Serotyping, Streptococcal Infections, Streptococcus agalactiae, Virulence Factors

We evaluated three different PCR-based capsular gene typing methods applied to 312 human and bovine Streptococcus agalactiae (group B Streptococcus [GBS]) isolates and compared the results to serotyping results obtained by latex agglutination. Among 281 human isolates 27% could not be typed by latex agglutination. All 312 isolates except 5 could be typed by the three PCR methods combined. Two of these methods were multiplex assays. Among the isolates that were typeable by both latex agglutination and capsular gene typing, 94% showed agreement between the two methods. However, each of the PCR methods showed limitations. One of the methods did not include all 10 recognized serotypes, one misidentified eight isolates of serotypes Ib and IV as serotype Ia, and one did not distinguish between serotypes VII and IX. For five isolates that showed aberrant patterns in the capsular gene typing, long-range PCR targeting the cps operon disclosed large insertions or deletions affecting the cps gene cluster. A sensitive flow cytometric assay based on serotype-specific antibodies applied to 76 selected isolates that were nontypeable by latex agglutination revealed that approximately one-half of these did express capsular polysaccharide. A procedure for convenient and reliable capsular gene typing to be included in epidemiological and surveillance studies of S. agalactiae is proposed.

Alternate JournalJ Clin Microbiol
PubMed ID23196363
PubMed Central IDPMC3553911