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International external quality assurance for laboratory identification and typing of Streptococcus agalactiae (Group B streptococci).

TitleInternational external quality assurance for laboratory identification and typing of Streptococcus agalactiae (Group B streptococci).
Publication TypeJournal Article
Year of Publication2011
AuthorsAfshar, B, Broughton, K, Creti, R, Decheva, A, Hufnagel, M, Kriz, P, Lambertsen, L, Lovgren, M, Melin, P, Orefici, G, Poyart, C, Radtke, A, Rodriguez-Granger, J, Sørensen, UBSkov, Telford, J, Valinsky, L, Zachariadou, L, Efstratiou, A
Corporate AuthorsMembers of the DEVANI Study Group
JournalJ Clin Microbiol
Volume49
Issue4
Pagination1475-82
Date Published2011 Apr
ISSN1098-660X
KeywordsBacterial Typing Techniques, Bacteriological Techniques, Europe, Female, Humans, Infant, Newborn, International Cooperation, Male, Molecular Typing, Pregnancy, Quality Assurance, Health Care, Serotyping, Streptococcal Infections, Streptococcus agalactiae
Abstract

We report the results from the first international multicenter external quality assessment (EQA) studies for molecular and serological typing of group B streptococcus (GBS) strains as part of DEVANI (Design of a Vaccine against Neonatal Infections), a pan-European program. A questionnaire-based surveillance was undertaken among eight laboratories participating in DEVANI and six laboratories not participating in DEVANI from 13 countries in order to assess their current microbiological procedures for GBS screening, diagnosis, and typing. GBS strains from three EQA distributions were characterized using molecular and serological methods based on GBS capsular polysaccharide typing. Participants were asked to test the first distribution using their current serotyping and genotyping methods. The Strep-B-Latex agglutination method was the most widely used method, with a typeability value of >90%. A multiplex PCR assay for GBS capsular gene typing was also used by 2 of 14 centers, which achieved a typeability value of 93%; this assay detected only 9 of 10 GBS capsular polysaccharide genes. From the second and third EQA studies, standardized protocols were prepared for serological and molecular typing of GBS strains based on the Strep-B-Latex agglutination method and a novel multiplex PCR assay that detected all 10 GBS capsular types (Ia to IX). These standardized protocols are being used by many European laboratories, and as the use of these methods increases, it is imperative to continuously improve and assess laboratory performance and offer training to any laboratories that have technical difficulties.

DOI10.1128/JCM.02365-10
Alternate JournalJ Clin Microbiol
PubMed ID21325542
PubMed Central IDPMC3122801