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Prevalence of , and -Carrying Plasmids in Isolated in a Brazilian Hospital.

TitlePrevalence of , and -Carrying Plasmids in Isolated in a Brazilian Hospital.
Publication TypeJournal Article
Year of Publication2021
AuthorsMigliorini, LB, de Sales, RO, Koga, PCM, Doi, AM, Poehlein, A, Toniolo, AR, Menezes, FG, Martino, MDV, Gales, AC, Brüggemann, H, Severino, P
JournalPathogens
Volume10
Issue3
Date Published2021 Mar 12
ISSN2076-0817
Abstract

carbapenemase (KPC) actively hydrolyzes carbapenems, antibiotics often used a last-line treatment for multidrug-resistant bacteria. KPC clinical relevance resides in its widespread dissemination. In this work, we report the genomic context of KPC coding genes , and in multidrug-resistant isolates from Brazil. Plasmids harboring and were identified. Fifteen additional carbapenem-resistant isolates were selected from the same tertiary hospital, collected over a period of 8 years. Their genomes were sequenced in order to evaluate the prevalence and dissemination of -harboring plasmids. We found that genes were mostly carried by one of two isoforms of transposon Tn (Tna or Tnb) that were predominantly located on plasmids highly similar to the previously described plasmid pKPC_FCF3SP (IncN). The identified pKPC_FCF3SP-like plasmids carried either or . Two isolates harbored pKpQIL-like (IncFII) plasmids, only recently identified in Brazil; one of them harbored in a Tna transposon. Underlining the risk of horizontal spread of KPC coding genes, this study reports the prevalence of and the recent spread of and , in association with different isoforms of Tn, together with high synteny of plasmid backbones among isolates studied here and in comparison with previous reports.

DOI10.3390/pathogens10030332
Alternate JournalPathogens
PubMed ID33809215
PubMed Central IDPMC7998191
Grant List2018/19243-4 / / Fundação de Amparo à Pesquisa do Estado de São Paulo /