Error message

Deprecated function: implode() [function.implode]: Passing glue string after array is deprecated. Swap the parameters in drupal_get_feeds() (line 394 of /var/www/

Proteome analysis of human sebaceous follicle infundibula extracted from healthy and acne-affected skin.

TitleProteome analysis of human sebaceous follicle infundibula extracted from healthy and acne-affected skin.
Publication TypeJournal Article
Year of Publication2014
AuthorsBek-Thomsen, M, Lomholt, HB, Scavenius, C, Enghild, JJ, Br├╝ggemann, H
JournalPLoS One
Date Published2014
KeywordsAcne Vulgaris, Bacterial Proteins, Hair Follicle, Humans, Inflammation, Propionibacterium acnes, Protein Interaction Maps, Proteome, Regeneration, Sebaceous Glands, Wound Healing

Acne vulgaris is a very common disease of the pilosebaceous unit of the human skin. The pathological processes of acne are not fully understood. To gain further insight sebaceous follicular casts were extracted from 18 healthy and 20 acne-affected individuals by cyanoacrylate-gel biopsies and further processed for mass spectrometry analysis, aiming at a proteomic analysis of the sebaceous follicular casts. Human as well as bacterial proteins were identified. Human proteins enriched in acne and normal samples were detected, respectively. Normal follicular casts are enriched in proteins such as prohibitins and peroxiredoxins which are involved in the protection from various stresses, including reactive oxygen species. By contrast, follicular casts extracted from acne-affected skin contained proteins involved in inflammation, wound healing and tissue remodeling. Among the most distinguishing proteins were myeloperoxidase, lactotransferrin, neutrophil elastase inhibitor and surprisingly, vimentin. The most significant biological process among all acne-enriched proteins was 'response to a bacterium'. Identified bacterial proteins were exclusively from Propionibacterium acnes. The most abundant P. acnes proteins were surface-exposed dermatan sulphate adhesins, CAMP factors, and a so far uncharacterized lipase in follicular casts extracted from normal as well as acne-affected skin. This is a first proteomic study that identified human proteins together with proteins of the skin microbiota in sebaceous follicular casts.

Alternate JournalPLoS One
PubMed ID25238151
PubMed Central IDPMC4169578